Today we ran our DNA samples that had been eaten with the nuclease and then stopped with the
EDTA through a DNA gel which measure how many
base pairs are present in the DNA. It does the by running a current through the gel and the very
negative DNA moves towards the positive end of the current. The smaller the
base pair number the faster the DNA moves so the higher
base pairs would be at the top of the gel and the lower at the bottom. Because it was running so slow we turned the voltage down and allowed the gel to run overnight, and tomorrow we are going to dye the gel and look at it with a UV light.
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