Well I started my day out by making more TBE buffer, which consist of 50 ml of TBE (Tris-Borate-EDTA buffer) and 450 ml of DI water. Once I was done making this buffer I made two DNA ladder, DNA ladder is a solution of DNA molecules of different known lengths and is used to run along side our samples in order to estimate the size of our samples. Once this was done, I prepared an extra micro-centrifuge tube with only 1 micro-liter of our chromatic supernatant, 9 micro-liters of 60% sucrose and 10 micro-liters of DI water. I made this extra sample in order to see how it looks on the gel without the trial digestion. For the solutions who already had already been through the trial digestion, I took 1 micro-liter from each and added them to new micro-centrifuge test tubes with the same numbers label in order to not get mix up. I also added 9 micro-liters of 60% sucrose and 10 micro-liters of DI water to the new test tubes. Once this was done I was ready to add them into my gel. The solutions on the gel can be seen in figure #1.
|Figure #1 Samples added to Gel|
|Figure # 2 Material needed for present and future usage|
|Figure #3 More beakers to make more Buffer|
When all of this was done and my gel was finally done, I took the gel and used a special UV light to the results. Unfortunately once again we didn't get the results we wanted. After I saw my results, I felt disappointed because I had worked so hard to get better results than last time but that didn't change the outcome. Like I said before in previous blogs, being a researcher is about working hard and trying to solve problems even if it means failing multiple times. It is hard to accept the results but my results just show me that I must work harder and try again until I get the results I want.